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KMID : 0364820170530030149
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Korean Journal of Microbiology
2017 Volume.53 No. 3 p.149 ~ p.155
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Filamentous growth of Escherichia coli by dephosphorylated NPr
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Choi Um-Ji
Seok Yeong-Jae
Lee Chang-Ro
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Abstract
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The nitrogen phosphotransferase (PTS) system is a regulatory cascade present in most Proteobacteria, where it controls
different functions. The nitrogen PTS is usually composed of EI Ntr (encoded by the ptsP gene), NPr (encoded by the ptsO
gene), and EIIA Ntr (encoded by the ptsN gene). While EIIA Ntr plays a role in a variety of cellular processes, such as potassium homeostasis, regulation of ppGpp accumulation, nitrogen and carbon metabolisms, and regulation of ABC transporters, little information is available for a physiological role of NPr. A recent study showed that dephosphorylated NPr affects adaptation to envelope stresses in Escherichia coli. In this study, we provide another phenotype related to NPr. The ptsP mutant showed a filamentation phenotype. The filamentation phenotype of the ptsP mutant was recovered by additional deletion of the ptsO gene, but not by additional deletion of the ptsN gene, suggesting that an increased level of dephosphorylated NPr in the ptsP mutant renders cells the filamentous growth. This idea was confirmed by the fact that cells with increased levels of dephosphorylated NPr shows the filamentation phenotype. Additionally, we showed that cell size of E. coli increases with incremental dephosphorylated NPr concentrations. These results suggested
that dephosphorylated NPr induces morphological change of E. coli.
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KEYWORD
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Escherichia coli, filamentous growth, nitrogen PTS, NPr
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